Course Details
Subject {L-T-P / C} : LS3095 : Life Science Minor Laboratory - I { 0-0-2 / 1}
Subject Nature : Practical
Coordinator : Prof. Sujit Kumar Bhutia
Syllabus
1. Detection and estimation of protein
2. Detection and estimation of carbohydrate
3. Enzyme assay
4. Microscopic examination of pure and mixed microbial cultures and serial dilution and spread plating of mixed microbial cultures
5. Streak plating, microscopy and gram staining techniques
6. Handling of liquid cultures and monitoring microbial growth phases via spectrophotometry
7. Observation of a plant cell.
8. Observation of an animal cell.
9. Observation of different phases of mitosis.
10. Observation of different phases of meiosis.
Course Objectives
- Students will gain hands on practice to estimate the biomolecules
- Students will gain hands on practice in basic microbiological experiments
- Students will do experiments to observe plant and animal cells
- Students will do experiments to observe mitosis and meiosis phases
Course Outcomes
After the completion of the syllabus, <br />Students will be able to estimate the biomolecules through spectrophotometer <br />Students will be able to culture and identify the shapes of the microbes <br />Students will be able to observe the plant and animal cells <br />Students will be able to observe the different phases of meiosis and mitosis
Essential Reading
- P. Hemalatha Reddy and Suman Govil, Life Sciences Protocol Manual, DBT, Government of India , E book compiled for Star College Scheme
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Supplementary Reading
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