Course Details
Subject {L-T-P / C} : LS5204 : RNAI and Oncogenomics { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Bibekanand Mallick
Syllabus
The concept of RNAi (RNA interference) and discovery, Gene silencing, Gene activation, other methods of gene silencing. Types, Biogenesis and Regulatory roles of non-coding RNAs - miRNA, siRNA, piRNA, lncRNA. RNAi-mediated gene silencing - Components and Mechanism, RISC and Proteins. The role of RNAi in development and differentiation. Oncogenesis, Tumour suppressor genes and Oncogenes. Cross-talk among ncRNAs and cancer, RNAi in oncogenesis and stem cells, Regulatory RNAs in cancer and cancer stem cells (CSCs), Types of stem cells, RNAi in induced pluripotency and cancer, Current research progress in RNAi therapeutics - case studies, significance and challenges.
Course Objectives
- The course will provide knowledge on recent advancement in the area of non-coding RNAs and their roles in diseases, especially cancer.
- The course will also cover basics of stem cells and their applications in regenerative therapy how to develop iPSCs using non-coding RNAs and other transcription factors.
Course Outcomes
After completion of the syllabus, students will be equipped to use the concept of RNAi and gene silencing as a useful tool for studying various biological problems, especially cancer and stem cells.
Essential Reading
- Bibekanand Mallick, Zhumur Ghosh, egulatory RNAs: Basics, Methods and Applications, 1st edition (2012), Springer-Verlag, Berlin Heidelberg, ISBN-9783642225161
- Brenner Charles (Ed.),, Oncogenomics: Molecular approaches to cancer, 2004, John Wiley, New Jersey, ISBN-9780471225928
Supplementary Reading
- Robert A Weinberg, The Biology of Cancer, 2nd edition (2013), W. W. Norton & Company 2nd edition ISBN-13: 978-0815342205
- Barik Sailen (Ed.),, RNAi: design and application, 2008, Humana Press, Totawa, ISBN-9781588298744