Course Details
Subject {L-T-P / C} : LS3302 : Bioinstrumentation { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Suman Jha
Syllabus
Principles, types and applications of pH meter, spectrophotometer/colorimeter, centrifuges, light-microscopes, sonicator, autoclave, biosafety cabinets/practices, electrophoretic techniques (including isoelectric focusing), precipitation, dialysis, ultrafiltration, lyophilization, polymerase chain reaction, and size exclusion chromatography.
Course Objectives
- Objective of the course is to make the life science students understand the basic rules and working principles of most basic instruments being used by biologists working in very different range of biological sciences.
Course Outcomes
Students shall be able to apply different instrumentation for their biological research, and shall be able to modify the technique for project’s suitability.
Essential Reading
- Keith Wilson, John Walker, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press , ISBN-978052173167
- Wester John G, Bioinstrumentation, Wiley & Sons , ISBN-97881265136
Supplementary Reading
- Plummer David T, Introduction to practical biochemistry, Tata McGraw Hill , ISBN-9780070994874
- Charles R Cantor, Paul R. Schimmel, Biophysical Chemistry (Techniques for the study of biological structure and function), (part II), W. H. Freeman and Company , ISBN-13: 978-0716711902