Course Details

Subject {L-T-P / C} : LS4191 : Biochemistry and Molecular Biology Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Prof. Samir Kumar Patra

Syllabus

1. Estimation of blood glucose and detection of (i) urea, (ii) uric acid, (iii) creatine, (iv)
creatinine, and (v) bile pigment in serum.
2. Determination of (i) sugar, (ii) ketone bodies, and (iii) protein in urine sample
3. Estimation of Hb concentration, total W.B.C. and R.B.C. count in blood.
4. Determination of lipid, total cholesterol, LDL, HDL and triglycerides.
5. Purification of serum albumin.
6. Enzyme assay and kinetics.
7. Protein fractionation – (i) Salting in and out, (ii) Gel filtration and affinity based
techniques, (iii) SDS-PAGE, and (iv) Electrophoretic separation of LDH isoenzymes.
8. Absorption and fluorescence spectroscopy related experiments.
9. Determination of CMC of biological surfactants.
10. Isolation of DNA from prokaryotic and eukaryotic cells.
11. Isolation of RNA from prokaryotic and eukaryotic cells.
12. Isolation of plasmid DNA from microorganisms.
13. Preparation of cDNA.
14. Gene expression analysis by Polymerase Chain Reaction.
15. Preparation of competent cells.
16. Cloning of external DNA into a plasmid vector.
17. Expression of foreign genes in Escherichia coli.

Course Objectives

Course Outcomes

• Students will be confident to explore and solve the disorders of life processes <br />• Students will be confident for executing and analyzing biological samples

Essential Reading

Supplementary Reading

Journal and Conferences