Course Details
Subject {L-T-P / C} : LS4104 : Molecular Biology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Samir Kumar Patra
Syllabus
The concept of chromosome: - Nucleic acids and histones. Mechanism of replication in prokaryotes and eukaryotes. Fidelity of replication, Inhibitors of DNA synthesis. Transcription – Mechanism and regulation in prokaryotes and eukaryotes, RNA polymerases, Promoters, Enhancers, Silencers, and Insulators. The concept of Operon, Intron, Exons and Cistrons. Post-transcriptional modifications, hn-RNA, sn-RNA and their role in the processing of RNA. Inhibitors of RNA synthesis. Translation - Genetic code and codon bias, Mitochondrial genetic code. Prokaryotic and eukaryotic ribosomes. Biosynthesis of Proteins – Amino acyls tRNA synthetases, mRNA, tRNA, Initiation, Elongation, and Termination. Inhibitors of translation, Post-translational modification and Sorting of proteins. Transposons and genetic recombination, Gene inactivation and Concept of epigenetics
Course Objectives
- What is the blue print of life?
- How information are passed
- What is gene, genome and gene expression
- What is epigenetics?
Course Outcomes
• Students will be able to decode the necessary signals programmed and stored in the chromosome. <br />• Students will be confident to explore and solve the disorders of life processes.
Essential Reading
- Alberts Bruce, Molecular biology of the cell, 5th edition (2008),, Garland Science, New York, , ISBN-9780815341062
- Lodish, Harvey, Molecular Cell Biology, 6th edition (2008),, WH Freeman, New York , ISBN-9781429203142
Supplementary Reading
- Elliott William H, Biochemistry and Molecular Biology, 3rd edition (2007), Oxford University Press, New York , ISBN-9780195672565
- Malacinski Georgem, Essentials of Molecular Biology, 3rd edition (1998), Jones and Bartlett Publishers, Boston , ISBN-867208600
Journal and Conferences
- Journal of Molecular Biology, J Biol Chemistry, FEBS J, BBA
- Cell, Nature, Science