Course Details
Subject {L-T-P / C} : CY5306 : Biochemistry { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Rabindra Kumar Behera
Syllabus
Basic cell biology: components of cells, cell organelle and their function, cell cycles and cell divisions, cell morphology, cell culture
Enzymes: Enzymatic reactions, Types of enzymes and their functions, Enzyme kinetics, Michaelis-Menten kinetics, reaction order, competitive, uncompetitive, non-competitive and irreversible inhibition of enzymes. Regulatory enzymes and their mechanism.
Bioenergetics: thermodynamic considerations, phosphoryl group transfers and ATP, redox potentials, bioenergetic principles.
Biosignaling: molecular mechanism of signal transduction, gated ion channels, sensory transduction in vision, olfaction, oncogenesis, tumor suppressor genes and programmed cell death.
Metabolism: aerobic and anaerobic oxidations, glycolysis, gluconeogenesis, pentose phosphate pathway, Photosynthesis, Calvin’s cycle, Kreb’s cycle, cofactors, mitochondrial electron transport and oxidative phosphorylation.
Fatty acid catabolism, amino acid oxidation and production of urea
DNA based information technologies: DNA cloning-the basics, from genes to genomes, from genomes to proteomes, concept of DNA microarray technology, genome alterations and new products of biotechnology, Genes and chromosomes: genetic recombination.
Course Objectives
- To learn how molecules of life behave in vivo for the survival of living organisms.
Course Outcomes
1) This course offers a thorough understanding of basic biochemical reaction. <br /> 2) The course helps to make the foundation for applying knowledge of chemistry for research in biology and medicine.
Essential Reading
- A. Lehninger, D. L. Nelson, M. M. Cox, Principles of Biochemistry, W. H. Freeman and Company , 5th Edition, 2008
- V. Voet and J. G. Voet, Biochemistry, John Wiley, New York , 2004
Supplementary Reading
- Jeremy M. Berg, Lubert Stryer, John Tymoczko, Gregory Gatto, Biochemistry, WH Freeman , 9th Edition, 2019
- Reginald H Garrett, Charles M Grisham, Biochemistry, Wadsworth Publishing Co Inc , 5th Edition, 2012