Course Details
Subject {L-T-P / C} : BM6531 : Advanced Bioseparation Technology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Kasturi Dutta
Syllabus
Basic review of Primary separation and recovery processes: Cell disruption methods for intracellular products, removal of insolubles, biomass (and particulate debris) separation techniques, flocculation and sedimentation, centrifugation and filtration methods. Ultracentrifugation as a separation technique for fractionation of cells and proteinsProducts isolation: Liquid-liquid Extraction and adsorption method, precipitation (ammonium sulphate. Organic solvents, high molecular weight polymers) Chromatographic separation affinity, size exclusion, Thin layer, ion exchange chromatography Modern separation: ultrafiltration, Reverse Osmosis, in situ product removal, integrated bioprocessing. Electrophoretic separation. Product finishing: precipitation/crystallization, mixing, dialysis, distillation and drying. Introduction to Process Analytical Technology (PAT) and Quality by Design (QbD) Scale down, monitoring and Validation of bioprocesses.
Course Objectives
- Advanced techniques of biosepartion in biotechnology
Course Outcomes
Students will learn to obtain pure products. <br />2. Students will learn to evaluate the product quality
Essential Reading
- P.A. Belter, E.L. Cussler and Wei-Houhu, Bioseparations – Downstream Processing For Biotechnology,, Wiley Interscience Pub
- R. K. Scopes, Protein Purification: Principles and Practice,, Springer
Supplementary Reading
- R Ghosh, Principles of Bioseparation Engineering, World Scientific Pte. Ltd,
- RG Harrison, PW Todd, SR Rudge. D Petrides, DP Petrides, Bioseparations Science and Engineering, Oxford University Press,