Course Details
Subject {L-T-P / C} : BM6444 : Genomics and Proteomics { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nandini Sarkar
Syllabus
Introduction and scope of proteomics and genomics Protein separation techniques Isoelectric focusing (IEF) Two dimensional PAGE for proteome analysis Image analysis of 2D gels Introduction to mass spectrometry Strategies for protein identification Protein sequencing Post translational modifications of proteins Quantitative proteomics Applications of proteome analysis to drug Protein-protein interaction (Two hybrid interaction screening) Protein chips and functional proteomics Clinical and biomedical application of proteomics Proteome database and data analysis Methods of preparing genomic DNA DNA sequence analysis methods: Sanger Dideoxy method and Fluorescence method Gene variation and Single Nucleotide Polymorphisms (SNPs) Expressed sequenced tags (ESTs) Gene-disease association Recombinant DNA technology: DNA cloning basics, Polymerase chain reaction, DNA fingerprinting, Human genome project and the genetic map.
Course Objectives
- Inferring the basic concepts of genomics and proteomics.
- Listing and discussing the use of genomics and proteomics in human health.
- Suggesting and outlining solution to theoretical and experimental problems in Genomics and Proteomics fields.
Course Outcomes
Application of basic knowledge of genomics and proteomics to solve theoretical and experimental problems in biotechnology and biomedical engineering.
Essential Reading
- R.M.Twyman, Principles of Proteomics, BIOS Scientific Publishers, 2004.
- Arthur M Lesk, Introduction to Genomics, Oxford University Press, 2007.
Supplementary Reading
- P. Michael Conn, Handbook of Proteomic Method, Humana Press, Totowa, New Jersay, USA, 2003.
- L. Stryer, Biochemistry, W. H. Freeman and Co., New York, 2007.