Course Details
Subject {L-T-P / C} : BM6402 : Advanced Protein Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nandini Sarkar
Syllabus
Introduction and scope of Protein Engineering Different approaches of protein engineering Random mutagenesis Mutagenesis by rational design Protein sequencing Effect of mutation on protein structure, stability and folding phi value analysis Applications of protein engineering Examples of commercially used engineered proteins Strategies for Protein identification Protein structural and biochemical characterization using fluorescence spectroscopy, circular dichroism, x ray crystallography, NMR, FTIR, mass spectrometry etc. Protein- protein interaction studies Protein post translation modifications and proteomics.
Course Objectives
- Introduction to different approaches of protein engineering
- Studies on effect of mutations on protein structure, stability and folding
- Studies on different methods of biophysical characterisation of proteins
- Examples of engineered proteins used in industrial, pharmaceutical and other applications
Course Outcomes
1. Gain in-depth knowledge on protein structure and physicochemical properties. <br />2. Review of factors significant for protein folding and stability. <br />3. Understanding of various methods of incorporating mutations in proteins and pros and cons of each method. <br />4. Understanding of various methods of protein purification and their underlying principle <br />5. Understanding of advanced biophysical techniques for protein analysis, including the capacity to discuss their relative merits and interpret data from those techniques.
Essential Reading
- G. Walsh, Proteins: Biochemistry and Biotechnology, Wiley Blackwell
- S. Park and J. Cochran, Protein Engineering and Design, CRC Press
Supplementary Reading
- K. D. Wittrup and G. L. Verdine, Methods in Enzymolgy- Protein Engineering for Therapeutics, Elsevier
- D. L. Purich and R. D. Allison, The Enzyme Reference, Academic Press