Course Details

Subject {L-T-P / C} : BM6471 : Advanced Cell and Molecular Engineering Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Prof. Bibhukalyan Prasad Nayak

Syllabus

Welcome to laboratory, introduction to laboratory equipment and learning safety and aseptic measures while working on wet bench/clean room.Basic principle of microscopy: hands on experience with light and inverted fluorescence microscope, working with magnification, resolution and visibility, aberrations, control of illumination in the microscope.Labeling techniques in fluorescence microscopy (labeling with antibodies, labeling with toxins and other specific molecules, labeling with substrate and ligand), Biological sample preparation and observation under TEM (pre-embedding and post-embedding methods).Fluorescent recovery after phot-bleaching (FRAP) to visualize MT organization.Cell viability assay using FACS.Isolation and purification of total RNA by adsorption and elution method.Amplification of DNA by polymerase chain reaction.DNA amplification and real time estimation using real time PCR device by SYBR green method.

Course Objectives

Course Outcomes

1. The students can carry out the wet lab experiments needed for cellular and molecular studies using latest protocols during their doctoral research work. <br />2. The students will get a clear idea regarding the engineering principles backing the operations of advanced biomedical research equipment.

Essential Reading

Supplementary Reading