Course Details
Subject {L-T-P / C} : BM6401 : Advanced Recombinant DNA Technology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Angana Sarkar
Syllabus
Recombinant DNA Technology: Relevance and impact of rDNA technology, enzymes used in gene manipulation, host systems and vectors : their relevance and applications, Gene isolation, cloning and expression, DNA sequencing, oligonucleotide synthesis, Southern and Northern hybridization, FISH, PCR, RAPD, RFLP, DNA fingerprinting and their applications for diagnosis of disease, site-directed mutagenesis, Gene silencing, Gene transfer technologies, Gene therapy Molecular basis of genetic diseases, genetic counseling. Functional genomics: DNA chips and microarray gene screen technology transgenic animals and gene knockout techniques. Genome sequencing projects, advancement of genome sequencing using Next generation Sequencing (NGS). Study of emerging genome editing tools including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system. Ethical clearances for recombinant products.
Course Objectives
- To gain detailed knowledge about molecular biology
- To learn the real application of RDT in different area
- To learn advancement of the recombinant DNA technology
- To learn ethical issues regarding recombinant products
Course Outcomes
Technical skills and knowledge development on versatile techniques in rDNA technology <br />Through understanding about the real applications of rDNA Technology <br />Gaining insight in the new advancements of the rDNA techniques and their field of application in real context <br />Expertise in designing and conducting experiments involving genome editing and manipulation <br />Development of awareness related to ethical issues of RDT
Essential Reading
- Brown TA, Gene cloning and DNA analysis, Wiley-Blackwell, USA.
- Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics., Wiley-Blackwell, USA.
Supplementary Reading
- Reece RJ, Analysis of genes and genomes, Wiley, USA
- Lewin B., Genes IX, Jones and Bartlett, USA