Course Details
Subject {L-T-P / C} : BM4107 : Fundamentals of Genomics,Transcriptomics and Proteomics { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Mukesh Kumar Gupta
Syllabus
Overview and general principles of omics technologies in biotechnology, Omics approaches: Genomics, Proteomics, Transcriptomics and Metabolomics, Meta-omics (genomics, proteomics, transcriptomics, metabolomics) and Functional omics for discovery of novel organisms, enzymes, value added products etc., Application of Omics in different fields of biotechnology including agriculture, environment, pharmaceuticals, medicine, forensic etc. Omic tools (DNA-sequencing, Genome Sequencing, Next Generation Sequencing(NGS) methods, gene expression microarray, MS-based proteomics, NMR-based metabolomics),ranscription factor binding sites,RNA-Seq, Microarrays, Regulatory RNAs: small or large, Computational prediction of miRNA target genes, RNA Darkmatter, SDS PAGE, 2D PAGE , Liquid chromatography , Mass Spectrometry (ESI and MALDI) ,Protein identification by peptide mass fingerprinting, Capillary electrophoresis, Gas chromatography, Electrochemical detector,Computational techniques in data integration and big-data handling inGenome Annotation, Omics projects worldwide.
Course Objectives
- • To develop knowledge on the basics of omics and their versatile applications.
- • Integration of omics approaches for improvement of life.
- • Technical skills and knowledge development on versatile techniques in omics
Course Outcomes
• Inferring the basic concepts of genomics, transcriptomics and proteomics. <br />• Listing and discussing the use of genomics and proteomics in human health. <br />• Suggesting and outlining solution to theoretical and experimental problems in Genomics, Transcriptomics and Proteomics fields.
Essential Reading
- • Barh D, Azevedo V, Omics Technologies and Bio-engineering: Towards Improving Quality of Life, Academic Press
- • Wittmann, C, Lee SY, Systems metabolic engineering, Springer Science & Business Media
Supplementary Reading
- • Pevsner J, Bioinformatics and Functional Genomics, Wiley-Blackwell , ISBN: 978-81-265-3834-8
- • Kihara, D, Protein function prediction for omics era, Springer Science & Business Media