Course Details
Subject {L-T-P / C} : BM4103 : Recombinant DNA technology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Angana Sarkar
Syllabus
Strategies of gene cloning, Tools in rDNA technology, Enzymes involved in RDT ( nuclease, Ligase, polymerases), Methods of construction of rDNAs,Vectors and their types, Introduction of rDNAs into living systems, Screening of rDNA clones, Application of RDT in different fields, PCR, RFLP, DNA fingerprinting and footprinting, Gene sequencing and mapping, In vitro gene expression systems, Transgenic system, Site-specific and oligonucleotide-directed mutagenesis, Antisense and ribozyme technology, Gene therapy, Ethical and legal issues in rDNA technology.
Course Objectives
- To gain knowledge about molecular biology
- To gain knowledge about application of molecular biology tools in real life
Course Outcomes
Enhanced knowledge about molecular biology <br />Enhanced knowledge and skill about application of molecular biology tools in real life
Essential Reading
- Brown TA, Gene cloning and DNA analysis, Wiley-Blackwell, USA.
- Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics., Wiley-Blackwell, USA.
Supplementary Reading
- Reece RJ, Analysis of genes and genomes, Wiley, USA
- Lewin B., Genes IX, Jones and Bartlett, USA