Course Details

Subject {L-T-P / C} : BM3700 : Tissue Engineering Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Prof. Devendra Verma

Syllabus

Module 1 - Scaffold Fabrication and Characterization Preparation scaffold biomaterial, fabrication of 3D porous scaffolds- Salt Leaching Method freeze drying, freeze gelation Methods Fabrication of nano fibrous scaffold by Electrospinning method Design of 3D scaffold by rapid proto typing technique. Characterization of biopolymers and scaffold-Mechanical Strength Contact angle measurement Pore size & Porosity Module 2 - Cells and Cell Culture Introduction to Cell Culture lab and aseptic skill (Use of Biosafety cabinet, CO2 incubators, Microscopes, Sterile Conditions) Preparation of Cell Culture Media and other supplements & Additives Isolation and Culturing of MNCs from Peripheral blood Cell counting & cell morphology, seeding of cells on scaffolds- static & dynamic method method Module-3-Cell Survival & Function Live/Dead Fluorescence Assay MTT Viability Test Cell Viability Test by Trypan Blue staining method

Course Objectives

Course Outcomes

The students will be able (i) to design and fabricate scaffold by various techniques (ii) to isolate and culture cells and learn the technique of making tissue constructs using scaffold and cells and (iii) to characterise the scaffold and construct.

Essential Reading

Supplementary Reading