Course Details
Subject {L-T-P / C} : BM4709 : Enzymology and Protein Engineering Laboratory { 0-0-2 / 1}
Subject Nature : Practical
Coordinator : Prof. Nandini Sarkar
Syllabus
Determination of Km and Vmax of an enzymatic reaction Effects of pH on enzymatic rate Effects of temperature on enzymatic rates Effects of substrate concentration on enzymatic rates ELISA Monitoring protein stability through equilibrium unfolding method Effects of pH on protein unfolding Effects of temperature on protein unfolding Monitoring protein folding/unfolding kinetics using stopped flow apparatus Structural characterization of proteins using various biophysical methods.
Course Objectives
- To study effects of external parameters like pH, temperature, substrate conc. on enzyme activity
- Protein folding studies using isothermal equilibrium method as well as kinetic studies.
- Structural characterisation of proteins using various biophysical methods
Course Outcomes
Students will be able to, <br />1. Plan and carry out activity measurements of enzymes and characterize their purity and stability. <br />2. Monitor effects of external factors as well as inhibitors on enzyme activity. <br />3. Use various biophysical methods for structural and biochemical analysis of proteins. <br />4. Monitor and understand effects of various environmental parameters on protein folding/unfolding.
Essential Reading
- G. Walsh, Proteins: Biochemistry and Biotechnology, Wiley Blackwell
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Supplementary Reading
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