Course Details
Subject {L-T-P / C} : BM4301 : Downstream Processing and Bioseparation { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Kasturi Dutta
Syllabus
Introduction to downstream processing, principles, characteristics of biomolecules and bioprocesses. Pretreatment and stabilisation of bioproducts. Unit operations for solid-liquid separation insoluble products: Centrifugation and Filtration: basic principles, design characteristics Ultracentrifuges: principles and applications Sedimentation Flocculation Cell disruption: Sonication Bead mills Homogenizers Chemical lysis Enzymatic lysis Separation of soluble products: Liquid-liquid extraction, aqueous two-phase extraction, Precipitation of proteins by different methods: salting in and salting out method, Adsorption. Membrane based purification: Ultrafiltration and Microfiltration Reverse osmosis Dialysis. Chromatography: principles, instruments and practice, adsorption, reverse phase, ion-exchange, size exclusion, hydrophobic interaction, bioaffinity and pseudo affinity chromatographic techniques. Electrophoresis. Final step: Drying, lyophilisation and crystallization in final product formulation.
Course Objectives
- Learn about fundamental downstream processes
Course Outcomes
Basic idea of fundamental and basic downstream processes
Essential Reading
- P.A. Belter, E.L. Cussler and Wei-Houhu, Bioseparations – Downstream Processing For Biotechnology,, Wiley Interscience Pub
- P. M. Doran, Bioprocess Engineering principles, Academic press.
Supplementary Reading
- M. L. Shular and F. Kargi, Bioprocess Engineering Basic concepts, Prentice Hall
- R.O. Jenkins, (Ed.), Product Recovery in Bioprocess Technology – Biotechnology, Open Learning Series, Butterworth-Heinemann