Course Details
Subject {L-T-P / C} : BM3101 : Genetic Engineering { 3-1-0 / 4}
Subject Nature : Theory
Coordinator : Prof. Mukesh Kumar Gupta
Syllabus
Understanding the gene, genome and DNA. Gene cloning and Vector: Importance of gene cloning and DNA analysis. Isolation of DNA: Purification of DNA from living cells, preparation of plasmid and bacteriophage DNA. Gene handling: Manipulation of purified DNA with various enzymes. Restriction and modification enzymes. Gene transfer: Introduction of DNA into living cells, Transformation, Identification of recombinants. Cloning Vector: Cloning vectors for prokaryotes and eukaryotes. Plasmids, bacteriophages, phagemids, cosmids, Ti plasmid, yeast artificial chromosome, Obtaining gene-specific clones: PCR, RT-PCR, real-time PCR and their various applications. Applications of gene cloning: Gene location, expression and function in animals. DNA labelling, DNA sequencing, DNA fingerprinting, Southern and Northern blotting, In-situ hybridization, RAPD, RFLP, Site-directed mutagenesis. cDNA and genomic DNA library. Genome function: Gene annotation, transcriptone and proteome. Production of recombinant proteins: Foreign gene expression in various systems. Genetic modification of animals: Animal bioreactor system, Production of bioactive phamaceutical proteins from animals. Gene targeting. Biopharaceuticals and Gene Therapy. Ethics in Genetic Engineering.
Course Objectives
- To impart training on genetic engineering of bacteria, mammalian cells, and animals.
- To choose and apply appropriate genetic engineering tool-box for manipulating of genes and genome.
- To design various gene cloning and expression strategies and their analysis.
- To understand and analyze biosafety, regulatory, ethical, and IPR issues with genetic engineering.
Course Outcomes
1. Apply the knowledge of genetic engineering on gene manipulation for industrial and societal applications. <br />2. Analyze, identify and apply appropriate genetic engineering toolbox for production of industrial proteins using recombinant DNA technology and genome editing for clinical applications. <br />3. Design suitable strategy for cloning of genetic elements for construction of expression vector. <br />4. Create and apply appropriate recombinant DNA technology for genetic analysis. <br />5. Exercise and make informed decisions on bio-safety and regulatory issues, ethics and IPR on genetic engineering.
Essential Reading
- TA Brown, • Gene Cloning and DNA analysis, Wiley-Blackwell, USA
- Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics, Wiley-Blackwell, USA , ISBN: 978-0632059546
Supplementary Reading
- Reece RJ, Analysis of genes and genomes, Wiley, USA , ISBN: 0-470-84379-9
- Lewin B, Genes IX, Jones and Bartlett, USA , ISBN: 978-0763740634