Course Details
Subject {L-T-P / C} : BM2702 : Bioprocess Engineering Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Kasturi Dutta
Syllabus
To prepare broth media for microbial culture, Microbial growth in shake flask. Kinetics of growth in batch cultivation- estimation of Monod kinetic parameters. Temperature effect on growth-estimation of energy of activation and Arrhenius Constant for microorganisms. A fed-batch and continuous culture in flask. Development of enzyme assays and quantification of enzyme activity and specific activity, Enzyme kinetics, Effect of pH and temperature on enzyme activity, Techniques of enzyme immobilization - matrix entrapment, ionic and cross linking
Course Objectives
- Overall idea about bioprocessing of products and its stability under different conditions
Course Outcomes
Basic overall idea for bioprocessing of products of industry relevance
Essential Reading
- K.R. Aneja, Experiments in Microbiology, Plant Pathology & Biotechnology, New Age International Publishers. 2007 , 4th edition
- P. Gunasekharan, Laboratory Manual in Microbiology, Newage International Publishers
Supplementary Reading
- J. Jayaraman, Laboratory Manaual in Biochemistry, New Age International Publications
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