Course Details
Subject {L-T-P / C} : BM2703 : Biochemistry and Microbiology Laboratory { 0-0-2 / 1}
Subject Nature : Practical
Coordinator : Prof. Devendra Verma
Syllabus
Biochemistry: Measurement of pH of biological liquids with pH-meter (ion meter) Quantitative
determination of iron ions in solution by a photoelectrocolorimetric method Quantitative
determination of serum protein by Bradford method using UV spectrophotometer Quantitative
determination of blood glucose by glucose oxidase method using semi -automated biochemical
analyzer Quantitative determination of urea in 24hr urine using semi-automated biochemical
analyzer and comparison with dipstick method.
Microbiology: Preparation of general purpose microbiological growth media using TSA/TSB
(tryptic soy agar/broth) Introduction to microscopy: to learn the principle and use of compound
light microscope Bacterial culture: to culturing bacteria by streak culture using aseptic
technique Simple staining: to stain bacteria under using safranin and crystal violet and
visualize under light microscope Gram staining: to learn gram staining protocol for classifying
the Gm+ve and Gm-ve bacteria.
Course Objectives
- To enable students learn basic level experiments related to medical biochemistry
- To enable students learn basic level experiments related to medical microbiology
Course Outcomes
The lab course will prepare the UG students ready for wet lab experiments involving estimating biochemical parameters and detecting micro organisms in human subjects.
Essential Reading
- Aparna B Verma, Practical Clinical Biochemistry, Jaypee
- Richard Goering, Mims' Medical Microbiology, Elsevier Health Sciences
Supplementary Reading
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