Course Details
Subject {L-T-P / C} : BM4003 : Analytical Techniques { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Angana Sarkar
Syllabus
At least any 10 experiments should be conducted from the following list:
Basic Techniques, Microscopy- Dark-field, Phase contrast, Fluorescence, Confocal, Polarization microscopy Electron microscopy: TEM & SEM, Radioisotope techniques- Basic concepts, GM and scintillation counter, autoradiography, RIA, Applications in biological science, Chromatographic methods- General principles, Ion exchange, Gel filtration, Affinity and Gas chromatography techniques, HPLC, Electrophoresis- General principles, Horizontal & Vertical Gel electrophoresis, Isoelectric focusing, 2D, Pulse field and immune-electrophoresis, Centrifugation techniques- Basic principles, Different types of centrifuges, Analytical and Preparative Ultracentrifugation methods, Spectroscopic Techniques- Electromagnetic radiations UV-Visible, fluorescence, CD, NMR, X-ray, Atomic absorption and Flame emission spectroscopic techniques, Mass spectrometry, Polymerase Chain Reaction, DNA sequencing, ELISA, Micro array, In-situ Hybridization
Course Objectives
- To know the basic principle of Biotechnology analytical techniques
- To learn about different equipment for biological sample analysis
Course Outcomes
Knowledge about different analytical techniques <br />Expertise development in equipment operation <br />Skill development in biological sample analysis
Essential Reading
- K. Wilson, and J. Walker, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press
- Pezzuto, John M., Michael E. Johnson, and Henri R. Manasse, Biotechnology and pharmacy, Springer Science & Business Media
Supplementary Reading
- I. D. Campbell, and R.A. Dwek, Biological Spectroscopy (Biophysical techniques series), Benjamin-Cummings Pub Cov
- J. A. Glasel, and M. P. Deutscher, Introduction to Biophysical Methods for Protein and Nucleic acid Research, Academic Press