Course Details
Subject {L-T-P / C} : BM6635 : Advanced Plant Tissue Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nivedita Patra
Syllabus
Conventional breeding Plant tissue culture for production of novel plants and hybrids: tissue culture media, callus and suspension culture, organogenesis, embryogenesis, protoplast culture, anther culture Cryopreservation: slow growth, DNA banking for germplasm conservation basic techniques of rDNA technology Plant transformation technology: tumor formation, hairy root, Ti and Ri plasmids, binary vectors, reporter gene with introns, particle bombardment, electroporation, microinjection, gene silencing Application of plant transformation Chloroplast transformation Metabolic engineering and industrial products: plant secondary metabolites, control mechanisms and manipulation of phenylpropanoid pathway, shikimate pathway, alkaloids, industrial enzymes, bioplastics, PHB, therapeutic proteins, antibiotics, edible vaccines, purification strategies Molecular marker-aided breeding: RFLP maps, linkage analysis, RAPD markers, STS microsatellite, SCAR, SSCP, AFLP, QTL, molecular marker assisted selection Arid plant biotechnology Green house technology.
Course Objectives
- To enable the students understand the basic technology for plant tissue culture.
Course Outcomes
1. To realize the significance of plant tissue culture in today life <br />2. To understand the plant tissue culture techniques <br />3. To comprehend the factors influencing various plant micropropagation studies <br />4. To appreciate the role of advanced biotechnology strategies in plant biotechnology
Essential Reading
- M.J. Chrispeels and D.E. Sadava, Plant, Genes and Crop Biotechnology, American Society of Plant Biologists , 2002
- S.S. Bhojwani and M.K. Razdan, Plant Tissue Culture: Theory and Practice, Elsevier Publishers , 1996
Supplementary Reading
- P.K. Chawla, Textbook of Plant Biotechnology, Oxford and IBH, New Delhi , 2002
- R. Verpoorte and A.W. Alfermann, Metabolic Engineering of Plant Secondary Metabolism, Kluwer Academic Publishers , 2000