Course Details
Subject {L-T-P / C} : BM6537 : Advanced Instrumentation in Bioengineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Kasturi Dutta
Syllabus
Sensing and measurement for quantitative molecular/cell/tissue analysis in terms of genetic, biophysical and biochemical properties Modern approaches to bioanalysis and bioassays using microscopic techniques like Light microscopy, Fluorescence microscopy, Atomic force microscope, Electron microscope, Scanning electron microscopy, Transmission Electron microsope Spectroscopic techniques like UV-Visible spectroscopy, Fluorescence spectroscopy, IR spectroscopy, CD spectroscopy, and Mass spectroscopy Principle, Design of horizontal and vertical gel electrophoresis apparatus, performing electrophoresis techniques, application of electrophoresis in analyzing macromolecules Chromatographic techniques Principles, Column chromatography, HPLC, TLC, Paper chromatography Qualitative analysis of biomolecules and cells using NMR, X ray crystallography, FACS etc.
Course Objectives
- basic working principles of analytical instruments related to biotechnological applications
Course Outcomes
Students will learn basic working principles of analytical instruments related to biotechnological applications <br />Students will also learn to analyse data obtained from these instruments.
Essential Reading
- 1. Manz, N. Pamme and D. Iossifidis, Bioanalytical Chemistry, World Scientific Publishing Company
- 2. D. L. Spector & R. D. Goldman, Basic Methods in Microscopy, Protocols and concepts from cells: A Laboratory Manual,, Cold Spring Harbor Laboratory Press
Supplementary Reading
- 1. R. D. Goldman, J. R. Swedlow and D. L. Spector, Live Cell Imaging: A Laboratory Manual, Cold Spring Harbor Laboratory Press
- H. H. Willard, Instrumental Methods of Analysis, CBS Publishers