Course Details
Subject {L-T-P / C} : BM6432 : Advanced Genetic Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Mukesh Kumar Gupta
Syllabus
Strategies of gene cloning, tools in rDNA technology, methods of construction of rDNAs, methods of introduction of rDNAs into living systems, screening of rDNA clones, PCR, RFLP, DNA fingerprinting and footprinting, chromosome walking, blotting techniques, gene sequencing and mapping, gene targeting, in vitro gene expression systems and protein engineering, transgenesis, metabolic engineering, examples of pathway manipulations, site-specific and oligonucleotide-directed mutagenesis, antisense and ribozyme technology, gene therapy, Genome Editing: ZFN, TELENS, CRISPR ethical and legal issues in rDNA technology.
Course Objectives
- • To impart training on fundamentals of exogenous gene expression using genetic engineering.
- • To choose appropriate genetic engineering tool-box for manipulating of genes and genome.
Course Outcomes
• Learn the available genetic engineering tool-box for manipulating of genes and genome. <br />• Understand the production of industrial proteins using recombinant DNA technology and genome editing for clinical applications. <br />• Learn and understand the bio-safety issues and regulatory bodies in genetic engineering. <br />• Understand ethical, legal and IPR issues with genetic engineering.
Essential Reading
- • Brown TA, Gene cloning and DNA analysis, Wiley-Blackwell, USA , ISBN: 978-1-4051-8173-0
- • Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics, Wiley-Blackwell, USA , ISBN: 978-0632059546
Supplementary Reading
- • Pinkert CA, Transgenic animal technology, Academic press, Amsterdam. The Netherlands
- • Cartwright EJ, ransgenesis techniques: Principles and Protocols. Methods in Molecular Biology 561, Springer Protocols, NY, USA