Course Details
Subject {L-T-P / C} : BM6131 : Analytical Techniques in Bioengineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Dr. Anju R. Babu
Syllabus
UV-visible spectroscopy (UV-Vis), Fourier transformed infrared spectroscopy (FT-IR), Mass spectroscopy(MS), Atomic absorption spectroscopy (AAS), MALDI, Nuclear magnetic resonance spectroscopy (NMR) Raman spectroscopy, High performance liquid chromatography (HPLC), Gas chromatography (GC),GC-MS and LC-MS, Spectrofluorimetry, Fluorescence recovery after photobleaching (FRAP), Fluorescence resonance energy transfer (FRET), Fluorescence correlation spectroscopy (FCS) , Circular dichroism (CD), Differential scanning calorimetry (DSC), Thermogravimetric analysis(TGA), X-ray diffraction analysis (XRD), X-ray photoelectron spectroscopy (XPS), Energy-dispersive X-ray spectroscopy(EDX), Surface profilometry and atomic force microscopy(AFM), Nanoindentation technique, Bright field and other optical microscopy techniques, fluorescence and confocal microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Zeta potential analysis, dynamic light scattering (DLS) Flow cytometry. Microarray, qPCR, Next-gen sequencing (NGS)
Course Objectives
- To give insight to the basic fundamentals of analytical techniques.
Course Outcomes
At the end of the course, the student will: <br />1. Learn the basic fundamentals of analytical techniques. <br />2. Identify the specific technique for the analysis of the biological analytes.
Essential Reading
- J.Cazes, Analytical Instrumentation Handbook, Third Edition, CRC Press , 2004
- R. Shobhaand M. Banani, Essentials of Analytical Chemistry, Pearson Education , 2017
Supplementary Reading
- Stanley Crouch, Douglas Skoog, Principles of Instrumental Analysis, David Harris , 2017
- R. S.Khandpur, Handbook of Analytical Instruments, McGraw-Hill Handbooks