Course Details
Subject {L-T-P / C} : BM6474 : Analytical Techniques in Bioengineering Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Kasturi Dutta
Syllabus
Quantitative estimation of biomolecules like proteins, nucleic acids, lipids, carbohydrates Determination of DNA melting temperature denaturant mediated protein unfolding using fluorescence spectrophotometer pH mediated protein unfolding using fluorescence spectrophotometer temperature mediated protein unfolding using fluorescence spectrophotometer effects of pH on enzyme kinetics effect of temperature on enzyme kinetics effect of substrate concentration on enzyme kinetics Chromatographic separation of biomolecules Protein ligand/nucleic acid ligand interaction studies using biophysical methods.
Course Objectives
- Techniques of studying protein interaction
- Analysis of biophysical properties of biomolecules
Course Outcomes
1. Students will learn techniques for studying interaction of proteins. <br />2. Students will acquire knowledge for analyzing different biophysical properties of biomolecule
Essential Reading
- J. Cazes, Analytical Instrumentation Handbook., CRC Press.
- , ,
Supplementary Reading
- , ,
- , ,