Course Details
Subject {L-T-P / C} : BM6644 : Animal Biotechnology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Mukesh Kumar Gupta
Syllabus
History and scope of animal biotechnology. Animal cell and tissue culture: Aseptic technique culture media buffer systems growth factors, supplements and substrates initiation and maintenance of cell cultures growth curve of animal cells in culture animal cell cryopreservation techniques immortalized culture and development of cell lines suspension culture perfusion and continuous flow cultures scaling up of animal cell cultures bioreactors for animal cell culture mass transfer in animal cell culture animal tissue culture techniques, organ culture. Animal genetic engineering: Introduction to cloning and gene technology, transfection of animal cell lines, Selectable and non-selectable markers, expression of cloned proteins in animal cells, vaccine development, development of Mabs. Transgenic animals - PN injection, viral vectors, SCNT, SMGT. Breed improvement: Economic traits quantitative traits upgrading progeny testing marker assisted selection estrous synchronization AI, superovulation, MOET, embryo splitting, ART, IVF. Animal biotechnology for in vitro and in vivo testing of drugs, toxicity testing of environmental pollutants, production of vaccines, pharmaceutical proteins and secondary metabolites. Bioethical issues related to animal biotechnology
Course Objectives
- • To understand the fundamentals of animal biotechnology for animal cell culture, and their genetic modification.
- • To apply biotechnology in manipulating animal genome and proteome for human welfare.
- • To learn the methods of transgenic animal production.
Course Outcomes
• Understand the application of biotechnology in manipulating animal genome and proteome for human welfare. <br />• Learn basics of cell and tissue culture, animal genetic engineering, animal transgenics, and assisted reproductive technologies in animals. <br />• Learn and understand the bio-safety issues and government regulations in animal biotechnology. <br />• Understand ethical, legal and IPR issues with animal biotechnology.
Essential Reading
- • Akbarsha A, Balakrishan K, Rajan KE, Animal Biotechnology, Pearson Education , ISBN: 978-8131787922
- • Freshney RI., Culture of Animal Cells, Wiley-Blackwell, USA , ISBN: 978-0-470-52812-9
Supplementary Reading
- • Hafez ESE and Hafez B, Reproduction in Farm Animals, Wiley-Blackwell, USA , ISBN: 978-0683305777
- • Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics, Wiley-Blackwell, USA , ISBN: 978-0632059546