Course Details
Subject {L-T-P / C} : BM6172 : Biomaterials and Tissue Engineering Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Amit Biswas
Syllabus
1. Preparation of Porous 3D Scaffold By Freeze Gelation Method
2. Preparation of Porous 3D Scaffold By Freeze Drying Method
3. Fabrication of Nanofibrous scaffold by Electrospinning
4. Swelling Study on Scaffolds
5. Degradation Study of 3D Porous Scaffold
6. Fabrication of 3D scaffold using Rapid Prototyping Technique
7. Contact Angle Measurement
8. Isolation of Mono-Nuclear Cells from Peripheral Blood
9. Cell Viability Assessment by Tryphan Blue Exclusion Assay
10. Cell Viability Assessment Using Fluoroscence Microscopy
11. Tensile Strength Determination of Electrospun Scaffold
12. Static Cell Seeding on Scaffold
13. Anodic oxidation of Ti-6Al-4V for medical application
14. Rheology of polymer solution using cone and plate method
15. Wearing of implant
Course Objectives
- Gains hands-on experience on various methods in biomaterials and tissue engineering
- Understanding different physical and chemical properties of the biomaterials required in tissue engineering.
- Fabrication, surface treatment and coating of different implant materials
- Understand working principles of various characterization techniques and protocols used in biomaterials and tissue engineering
Course Outcomes
-Demonstrate laboratory skills in fabrication and characterization of scaffolds for tissue engineering. <br />-Able to understand the need of different characterization techniques. <br />-Able to explain how composition, structure and treatment modify the mechanical properties. <br /> -Able to describe the interactions of biomaterials with the biological environment <br />-Learn aseptic techniques in handling cell culture and tissue engineering.
Essential Reading
- -Wujing Xian, -A Laboratory Course in Biomaterials, -CRC Press , -
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Supplementary Reading
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