Course Details
Subject {L-T-P / C} : LS4391 : Bioanalytical Techniques Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Suman Jha
Syllabus
Syllabus contain a series of 8-10 experiments to explore the structure biology.
1.Preparation of different solution (protein, lipid, nucleic acid) for FTIR, UV-Visible, Fluorescence spectroscopic measurements.
2.DNA melting and reannealing study using UV-Visible spectroscope.
3.Study of conformational change in protein using FTIR/CD spectroscope.
4.Effect of different solvent on fluorescence property of intrinsic/extrinsic fluorophore using fluorescence spectroscope.
5.Preparation of different acid/base dilution to differentiate between activity and concentration.
6.Isolation of protein from microbe/plant/animal cells using electrophoresis.
7.Purification of protein using size exclusion chromatography.
8.Determination of concentration of sugars using HPLC.
Course Objectives
- To make post-graduate life science students independent in executing different experiments of structural biology and analytical sciences (for biomacromolecules).
Course Outcomes
Student will be able to characterize the presence of different biomacromolecules in sample (unknown) and its properties like thermodynamical and enzymatical properties.
Essential Reading
- Keith Wilson, John Walker, Practical Biochemistry (Principles and techniques), Cambridge University Press , x
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Supplementary Reading
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