Prof. Surajit Das, Neelam Mangwani, Supriya Kumari, S. K. Shukla
and T. S. Rao, Department of Life Science, Phenotypic switching in biofilm-forming
marine bacterium Paenibacillus lautus NE3B01.
iofilm forming marine bacterium Paenibacillus lautus NE3B01 formed a swarming type
of colony pattern and revealed the existence of two phenotypicaly distinct cells
(i.e. cocci and rods). Biofilm growth studies (crystal violet assay) with the isolate showed
| significant biofilm formation by 6 h and the detachment phase was observed
after 18 h. The CSLM study showed that P. lautus formed a biofilm with a average
thickness of 14.8 ± 2.6 µm; a high roughness coefficient (0.379 ± 0.103) and surface
to bio-volume ratio (4.59±1.12 µm2/ µm3) indicating a highly uneven topography of
the biofilm. The current findings suggest that P. lautus has two vegetative phenotypes
and can switch over from motile rods to nonmotile cocci and vice-versa. The work
is supported by Department of Biotechnology, Government of India. More in Current
Microbiology (2014) 68: 648-656. DOI 10.1007/s00284-014-0525-8.
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