Course Details
Subject {L-T-P / C} : BM2315 : Biochemistry and Microbiology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nandini Sarkar
Syllabus
The foundations of biochemistry- Cellular and chemical foundations of life, Introduction to Biomolecules – Chemical Composition and Reactivity, Macromolecules and their monomeric subunits, Amino Acids, Proteins, Lipids, Carbohydrates, Nucleic Acids, Enzyme kinetics, Enzyme Inhibition, Metabolism of carbohydrate and lipids, Concepts of protein and nucleic acid sequencing, History of microbiology and notable contribution of eminent scientists, Classification of microorganisms: bacteria, virus, protozoa, algae, fungi, Ultrastructure of prokaryotes and eukaryotes, Gram staining, Cultivation, preservation and control of microorganisms: Culture characteristics and Growth pattern, Nutritional and Physical Requirements, Types of media, Isolation, Maintenance and Preservation of the pure cultures, Applications of microbes in different fields including medicine, environment, food and fermentations, dye, energy etc.
Course Objectives
- To identify the different classes of polymeric biomolecules and their monomeric building blocks.
- To explain how the metabolism of biomolecules (carbohydrates and lipids) leads ultimately to the generation of energy.
- To explain the specificity of enzymes (biochemical catalysts) and the chemistry involved in enzyme action and inhibition.
- To gain detailed knowledge about different microorganisms and to explain how microbes grow in laboratory condition, how their growth can be controlled and their applications.
Course Outcomes
1. To have in-depth understanding of the structure and physicochemical properties of important biomolecules. <br />2. To demonstrate an understanding of fundamental biochemical principles, such as the structure/function of biomolecules, metabolic pathways, and the regulation of biological/biochemical processes. <br />3. To describe the classification and mechanism of enzyme action and inhibition. <br />4. To know about the identification and characterization process of microorganisms <br />5. To gain insight about microbial, preservation techniques and their applications in various fields.
Essential Reading
- Nelson D and Cox, M., Lehninger: Principles of Biochemistry, Freeman and Company
- Atlas, RM., Principles of Microbiology, McGraw-Hill
Supplementary Reading
- Voet D, Voet JG., Biochemistry, Willey
- Pelczar. M., et al, Microbiology, McGraw-Hill