Course Details
Subject {L-T-P / C} : BM6535 : Metabolic Regulation and Engineering { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Nandini Sarkar
Syllabus
Introduction to metabolic engineering: Overview to the field with illustrating examples. Central Metabolism: Fueling metabolism, Supply of biomass precursors, Anabolism, Anaplerosis. Coordination of metabolic reactions: Feedback inhibition, Energy charge, Multigene networks. Methods for metabolic characterization: Genome, Transcriptome, Proteome, Metabolome, Fluxome.Comprehensive models for cellular reactions: Stochiometry of cellular reactions, Reaction rates, Dynamic mass balance. Regulation of metabolic pathways: Regulation of Enzymatic Activity, Regulation of Enzyme concentration, Regulation at whole cell level, Regulation of Metabolic networks.Metabolic flux analysis: Overdetermined and undetermined systems, Sensitivity analysis. Methods for Metabolic Flux Analysis - Metabolite Balancing, Tracer Experiments, MS and NMR in labelling measurement.Applications of metabolic flux analysis.Metabolic control analysis (MCA): Determination of Flux control coefficients, MCA of Linear and Branched pathways. Metabolic design: Gene amplification, Gene-disruption, Randomized and targeted strain development. Metabolic Engineering in Practice: Actual examples from research and industrial biotechnology.
Course Objectives
- Knowledge of stoichiometry and energetics of metabolism.
- To apply practical applications of metabolic engineering in chemical, energy, medical and environmental fields.
Course Outcomes
Application of knowledge of metabolic engineering in chemical, energy, medical and environment fields.
Essential Reading
- Stephanopoulas, G, et al, Introduction to Metabolic engineering – Principles and Methodologies, Elsevier Science
- S. Y. Lee & E.T. Papoutsakis, Metabolic Engineering, Marcel Dekker, New York, 1999
Supplementary Reading
- R. Heinrich and S. Schuster, The Regulation of Cellular Systems, Chapman & Hall, 1996
- E.O. Voit, Computational Analysis of Biochemical Systems, Cambridge University Press, 2000.