Course Details
Subject {L-T-P / C} : BM3002 : Analytical Techniques in Biotechnology { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Prof. Kunal Pal
Syllabus
Basic techniques in Biotechnology: Microscopy: The light microscope. ? Phase contrast, confocal, Electron microscopy: TEM & SEM, Mass spectrometric techniques: Introduction, Ionisation, Mass analysers, Detectors, Structural information by tandem mass spectrometry, MALDI-TOF, Electrophoretic techniques General principles, Support media, Electrophoresis of proteins, Electrophoresis of nucleic acids, Capillary electrophoresis, Microchip electrophoresis. Chromatographic techniques: Principles of chromatography, Chromatographic performance parameters, High-performance liquid chromatography, Adsorption chromatography, Partition chromatography, Ion-exchange chromatography, Molecular (size) exclusion chromatography, Affinity chromatography, Gas chromatography. Spectroscopic techniques: Ultraviolet and visible light spectroscopy, Fluorescence spectroscopy, Luminometry, Circular dichroism spectroscopy, Light scattering, Atomic spectroscopy, Infrared and Raman spectroscopy, Surface plasmon resonance, Electron paramagnetic resonance, Nuclear magnetic resonance, X-ray diffraction
Course Objectives
- The primary objective of the course is to familiarize the students with the basic principles and theory of instrumental analysis.
- The student will gain an insight into the key methodologies employed during analytical analyses
- The students will gain knowledge to interpret results obtained from analytical instruments.
- The students will be able to solve mathematical problems that are related to the use of the analytical instruments
Course Outcomes
At the end of the course, the students will be able to: <br />1. categorize different analytical techniques on the basis of their operational principle <br />2. understand how to apply the basic principles of analytical techniques in handling the analytical instruments <br />3. design basic analytical instruments and tailor the specifications of different analytical instruments <br />4. determine unknown concentrations and obtain structural information of analytes <br />5. understand the strengths, limitations, and use of the analytical techniques for problem-solving
Essential Reading
- K. Wilson, and J. Walker, Principles and Techniques of Biochemistry and Molecular Biology, Cambridge University Press
- D. Campbell, and R.A. Dwek, Biological Spectroscopy (Biophysical techniques series), Benjamin-Cummings Pub Co
Supplementary Reading
- Irwin Segel, Biochemical calculations, Wiley and Sons
- J. A. Glasel, and M. P. Deutscher, Introduction to Biophysical Methods for Protein and Nucleic acid Research, Academic Press