Course Details
Subject {L-T-P / C} : BM3711 : Genetic Engineering Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Prof. Mukesh Kumar Gupta
Syllabus
Equipments used in genetic engineering. Construction of gene cloning and gene expression vectors. Purification and Quantification of nucleic acids for cloning and ectopic gene expression. Gene handling: Restriction enzyme mapping. Gene Transfer: Preparation of competent cells, Transformation, Identification of recombinants. How to obtain a clone of a specific gene: Primer designing, Degenerate primers, PCR (simplex, duplex and multiplex), Gradient PCR. Gene expression analysis: RT-PCR, Semi-quantitative PCR, Quantitative PCR. Gene and protein function: Annotation and sequence analysis of nucleotide and peptide sequences.
Course Objectives
- • To impart training on use of various genetic engineering tool-box for manipulating of genes and genome.
- • To learn the various gene expression strategies and their analysis.
Course Outcomes
• Learn the available genetic engineering tool-box for manipulating of genes and genome. <br />• Learn the production of industrial proteins using recombinant DNA technology.
Essential Reading
- • Williams SA, Slatko BE, McCarrey JR, Laboratory Investigations in Molecular Biology, Jones and Bartlett Publishers, USA , ISBN-13:978-0-7637-3329-2
- • Green MR, Sambrook J, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press , ISBN: 978-1-621821-04-5
Supplementary Reading
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