Environmental biotechnology, Marine microbiology, Aquaculture, Fish biotechnology, Biodiversity & ecology
DGGE, based on partial DNA strand separation at a given position in a gradient of chemical denaturant, are described, and an example protocol, optimized for fingerprinting of 200-300 bp fragments of bacterial 16S rRNA genes, is given.
A GC (guanine plus cytosine) rich sequence can be incorporated into one of the primers used in the PCR to modify the melting behaviour of the fragment of interest and to improve the separation of the fragments.