Course Details
Subject {L-T-P / C} : LS5304 : Genomics and Proteomics { 3-0-0 / 3}
Subject Nature : Theory
Coordinator : Sujit Kumar Bhutia
Syllabus
| Module 1 : |
Genome organization-prokaryotic and eukaryotic genomes, Genome mapping, assembly, and comparative genomics, DNA sequencing technologies- Sanger, Next-Generation Sequencing, Genome Annotation and analysis |
| Module 2 : |
Functional genomics-transcriptomics, gene expression profiling, Microarray, RNA-seq, Applications in personalized medicine, genome editing, agriculture, Ethical, legal, and social issues in genomics |
| Module 3 : |
Structural and functional proteome, proteomics workflow, sample preparation for proteomics, insoluble proteomics, defining proteomics experiments, minimizing dynamic range, Protein separation-1D/2D gel electrophoresis, Isoelectric focusing, chromatography techniques |
| Module 4 : |
Protein identification and Quantification-Mass spectrometry, MALDI-TOF, ESI-MS/MS, Sequence analysis, Protein-protein interactions for target identification, Clinical and applied proteomics, biomarker discovery, disease proteomics |
Course Objective
| 1 . |
To understand genome organization, gene expression, and proteome complexity |
| 2 . |
To familiarize students with modern genomic and proteomic technologies and analytical techniques |
| 3 . |
To develop the ability to interpret genomic, transcriptomic, and proteomic data in biological research |
| 4 . |
To apply genomics and proteomics knowledge to biomedical, agricultural, and biotechnological applications |
Course Outcome
| 1 . |
Students understand the principles of genome structure, gene regulation, and proteome diversity |
| 2 . |
Students would be able to utilize genomic and proteomic techniques for data generation and analysis |
| 3 . |
Students develop the skill to interpret genomic, transcriptomic, and proteomic datasets using appropriate tools |
| 4 . |
Students develop expertise on genomics and proteomics concepts to research, health, agriculture, and biotechnology |
Essential Reading
| 1 . |
Terry A. Brown, Genomes 5, CRC Press , https://doi.org/10.1201/9781003133162 |
| 2 . |
Julie D. Thompson, Christine Schaeffer-Reiss, Marius Ueffing , Functional Proteomics: Methods and Protocols , Humana Press , https://doi.org/10.1007/978-1-59745-398-1 |
Supplementary Reading
| 1 . |
Devarajan Thangadurai, Jeyabalan Sangeetha , Genomics and Proteomics: Principles, Technologies, and Applications , CRC press , https://doi.org/10.1201/b18597 |
| 2 . |
Rakeeb Ahmad Mir, Sheikh Mansoor, Sajad Majeed Zargar, Principles of Genomics and Proteomics , Academic Press , ISBN: 9780323993784 |
Journal and Conferences
| 1 . |