Syllabus

1. Estimation of blood glucose and detection of (i) urea, (ii) uric acid, (iii) creatine, (iv)
creatinine, and (v) bile pigment in serum.
2. Determination of (i) sugar, (ii) ketone bodies, and (iii) protein in urine sample
3. Estimation of Hb concentration, total W.B.C. and R.B.C. count in blood.
4. Determination of lipid, total cholesterol, LDL, HDL and triglycerides.
5. Purification of serum albumin.
6. Enzyme assay and kinetics.
7. Protein fractionation – (i) Salting in and out, (ii) Gel filtration and affinity based
techniques, (iii) SDS-PAGE, and (iv) Electrophoretic separation of LDH isoenzymes.
8. Absorption and fluorescence spectroscopy related experiments.
9. Determination of CMC of biological surfactants.
10. Isolation of DNA from prokaryotic and eukaryotic cells.
11. Isolation of RNA from prokaryotic and eukaryotic cells.
12. Isolation of plasmid DNA from microorganisms.
13. Preparation of cDNA.
14. Gene expression analysis by Polymerase Chain Reaction.
15. Preparation of competent cells.
16. Cloning of external DNA into a plasmid vector.
17. Expression of foreign genes in Escherichia coli.

What is need of Practical Biochemistry and Molecular Biology?

Forensic laboratory

Clinical biochemistry

Basics of experimental research

• Students will be confident to explore and solve the disorders of life processes
• Students will be confident for executing and analyzing biological samples

Nelson David L, Lehninger principles of biochemistry, 5th edition (2008), W H Freeman & Company , ISBN-9781429208925

Sawhney SK, Introductory practical biochemistry, 1996, Narosa, New Delhi, , ISBN-8173193029

Campbell MK, Biochemistry, 5th edition (2009), Cengage Learning, Australia, , ISBN-9788131502839

Cell, J Experimental Medicine, Exp Cell Res, Epigenetics

Journal Clinical Investigation, Analytical Biochemistry