Syllabus

1. To study the guidelines for working in microbiology laboratory.
2. To study various aseptic techniques used in microbial experiment.
3. Isolation of total heterotrophic bacteria from soil and water.
4. Enumeration of air-borne microorganisms.
5. Isolation of pure culture.
6. To perform Gram’s staining to distinguish Gram-positive and Gram-negative bacteria.
7. Simple staining of bacteria and fungi.
8. Growth phase and growth curve of bacteria.
9. Antibiotic sensitivity test of microbes.
10. Determination of Minimum Inhibitory Concentration (MIC) of antibiotics.
11. To test starch hydrolysis by amylase activity.
12. To study bacterial motility.
13. To study bacteriology of water by Most Probable Number (MPN) method.
14. To study principle and procedure of IMViC test.
15. To study microbial catabolic gene expression.
16. Bio-reporter assay for screening biofilm forming bacteria
17. Qualitative and quantitative assessment of bacterial biofilm

To learn the basic to advanced hands-on laboratory experiments

After studying this course the students will be able to do microbiological experiments independently.

Verma, A. S., S. Das and A. Singh, https://www.amazon.in/Laboratory-Manual-Biotechnology-Students-Ashish-ebook/dp/B06XKS5421, S. Chand Publishers

James G. Cappuccino, Microbiology: A Laboratory Manual, Peaarson