Syllabus

Module I: Spectroscopic Techniques – UV-visible spectroscope, Fluorescence Spectroscope, Optical Rotatory Dispersion, Circular Dichroism Spectropolarimeter, Fourier Transform InfraRed Spectroscope, Raman Spectroscope.
Module II: Calorimeter – Differential Scanning Calorimeter and Isothermal Titration Calorimeter.
Module III: Separation Techniques – Chromatography (all separation principles of chromatography) Isoelectric-focusing ESI/MALDI-TOF-MS.
Module IV: Microscopes – Fluorescence microscope, Confocal Laser Scanning Microscope, Atomic Force Microscope, Scanning/Transmission Electron Microscope.
Module V: Nuclear Magnetic Resonance spectroscope.

The main objective, in brief, of the course is to make the students understand different analytical tools employed by pharmaceutical/biotechnological industries and academic groups working in biological sciences. Hence, the specific objectives of the course are to:
1. Familiarize the students with basic principles and theory of analytical instruments-based analysis,

2. Understand different thermodynamic parameters’ role in governing different structures (molecular/population heterogeneity) to biomolecules,

3. Impart knowledge necessary to design and interpret the experiment's outcome using studied analytical tools.

After completion of the course, the student will be able to:
? Categories different studied techniques as per their underlying principle and utilize them for designing the experiment/project,
? Tailor different specifications of the instrument as per the needs of the project
? Understand the strengths and limitations of the techniques and use them in the design, execution, and interpretation of the outcomes from the experiment.

Keith Wilson, John Walker, Practical Biochemistry (Principles and techniques), Cambridge University Press , x

Cantor, Schimmel., Biophysical Chemistry (part I, II, and III)., W H Freeman , x

Arrondo JLR, Alonso A (Eds.), Advanced Techniques in Biophysics, Springer (Series in Biophysics) , x

Talluri Sekhar, Bioanalytical Techniques, IK International Publishing House, New Delhi, , ISBN-9789381141700