Syllabus

Module # 1
Introduction: (2-3 hours)
• Introduction to Bio-inorganic Chemistry
• Metal ions essential for biological functions: The Inorganic Side of Life
• Basic coordination chemistry in biology: Biological ligands for metal ions.
• Metal ion specificity/selectivity.
Ion-transport and Signal transduction: (4 hours)
• Ion (Na+ and K+) transport: Ion Channels & Pump, Ionophore, Action Potential
• Ca2+: Cell Signalling (Calmodulin) and Heart Pumping (Troponin C)
• Mg2+: ATP Hydrolysis

Module # 2
O2 storage and transport: (4 hours)
• Myoglobin/Hemoglobin and Hemocyanin (Cu)/Hemerythrin [Resonance Raman Spectra]
O2 activation and toxicity/detoxification: (3 hours)
• Cytochrome P450, Peroxidase, Catalase, Super Oxide Dismutase (SOD)
Fe transport, storage and acquisition: Transferrin, Ferritin and Siderophore (2 hours)

Module # 3
Electron Transfer: Cytochromes, Fe-S Cluster, and Plastocyanin (Cu) Marcus Theory (2 hours)
Copper Proteins (Types): Electron transport and O2 activation (1 hour)
ATP Synthesis: Photosynthesis, and Respiration: Cytochrome C Oxidase (Fe & Cu) (4 hours)
Nitrogen Fixation: Nitrogenase (Fe & Mo) (1 hour)

Module # 4
Zn2+ (Lewis Acidity): Carbonic Anhydrase, Carboxy Peptidases, ADH and Zinc Finger protein (2 hours)
Group Transfer and Pernicious/Megaloblastic Anaemia: Vitamin B12 (Co) (1-2 hours)

Module # 5
Metals in Health: (3 hours)
• Metal toxicity: Mercury and Arsenic Metals in Diagnosis: Gd, Fe Metals in Treatments: Pt, Au
Methods in Bio-inorganic Chemistry: (4 hours)
• Resonance Raman, Mössbauer and EPR Spectroscopy, Cyclic Voltammetry, Site-Directed Mutagenesis

To study the “Inorganic Side of Life”.

To study the “Metal ion Selectivity” by nature in molecules of life.

To study how metalloenzymes achieve extraordinary reactivity by suitable primary/secondary coordination environment.

CO 1: It provides idea about essentiality of inorganic element required for life and their bioavailability.
CO2: It teaches about – how metalloproteins select a metal ion (over another) for its structure/activity.
CO3: The course provides the insight about - how metalloenzymes functions (i.e. mechanism – structure/activity relationship) to regulate further for potential applications.
CO4: It provides information about the metal ions associated with physiology and pathophysiology (Metal ions in functionality/toxicity to diagnosis to treatment).
CO5: It provides a brief idea about the analytical methods to study the metal coordination/binding site and reactivity.

Bertini, I. Gray, H. B. Stiefel, E. I. Valentine, J. S., Biological Inorganic Chemistry: Structure and Reactivity, University Science Books , 2007

Robert Chrichton, Biological Inorganic Chemistry: A New Introduction to Molecular Structure and Function, , Elsevier , 2012

Wolfgang Kaim and Axel Klein, Bio-inorganic Chemistry, Wiley , 2013

L. Que. Jr., Physical Methods in Bioinorganic Chemistry, University Science Books

Video Lectures by Dr. R.K. Behera A.N. Koshla Center for Technology Enabled Learning: https://ankctel.nitrkl.ac.in/lecture/13

JBIC, Metallomics, JBC, JIB