Syllabus

Module 1: Introduction to Enzymes (10 Contact Hours)
Enzymes, general characters, biological vs chemical catalysis, nomenclature, Structure and function of enzymes, Structural classification, enzyme denaturation - renaturation Specificity, Enzyme structural components, Enzyme activity, Turnover number, Specific Activity. RNA Enzymes. Enzyme catalysis, Initial velocity studies, Estimation of Michaelis Menten parameters, Effect of pH and temperature on enzyme activity, kinetics of inhibition.
Module 2: Enzyme Immobilization (6 Contact Hours)
Techniques of enzyme immobilization kinetics of immobilized enzymes, effect of solute, partition & diffusion on the kinetics of immobilized enzymes, design and configuration of immobilized enzyme reactors applications and economics of immobilized enzyme technology.
Module 3: Production of Enzymes (7 Contact Hours)
Raw material, pretreatment methods, choice of microorganisms, fermentation methods: solid state fermentation, submerged fermentation, Other production methods. Cloning, Expression in Host and Extraction Strategies.
Module 4: Purification of Enzymes (8 Contact Hours)
Introduction, traditional enzyme purification methods, Ion exchange chromatography, Gel filtration, Bio-affinity Chromatography, Hydrophobic interaction chromatography, Hydroxyapatite Chromatography, Enzyme purification protocol. Purification of Recombinant Enzymes: Immobilized Metal Affinity Chromatography, Affinity chromatography with protein tags
Module 5: Use cases for Industrial Applications (6 Contact Hours)
Production and purification of large-scale enzyme in the area of: medicine, food, environment, and lifestyle.

To introduce the catalytic mechanisms of enzyme reactions, with an emphasis on: cofactors in enzymology, mechanisms of group transfer reactions and oxido-reduction reactions

To realise the various applications of enzymes in the fields of food industry, pharmaceutical industry and other applications

1. Students will be able to learn about enzyme structure, function and enzyme catalyzed reactions
2. Students will be able to understand engineering principles for enzyme production at large scale.
3. Students will be able to design a production and purification strategy for a hypothetical enzyme of choice.

Najafpour, G.D.,, Biochemical Engineering and Biotechnology, Elsevier Publishers , 2015

Katoh, S., J. Horiuchi and F. Yoshida,, Biochemical Engineering: A Textbook for Engineers, Chemists and Biologists, Wiley publications , 2016

Wolfgang, A.,, Enzymes in Industry: Production and Applications, Wiley publications , 2007

NIIR Board of Consultants & Engineers, Enzymes Biotechnology Handbook, Asia Pacific Business Press, India , 2004