Syllabus

Module: 1 Introduction to metabolic engineering: Overview to the field with illustrating examples. Central Metabolism: Fueling metabolism, Supply of biomass precursors, Anabolism, Anaplerosis. Coordination of metabolic reactions: Feedback inhibition, Energy charge, Multigene networks. (10 lectures)

Module 2: Methods for metabolic characterization: Genome, Transcriptome, Proteome, Metabolome, Fluxome.Comprehensive models for cellular reactions: Stochiometry of cellular reactions, Reaction rates, Dynamic mass balance. Regulation of metabolic pathways: Regulation of Enzymatic Activity, Regulation of Enzyme concentration, Regulation at whole cell level, Regulation of Metabolic networks. (10 lectures)

Module: 3 Metabolic flux analyses: Overdetermined and undetermined systems, Sensitivity analysis. Methods for Metabolic Flux Analysis - Metabolite Balancing, Tracer Experiments, MS and NMR in labelling measurement.Applications of metabolic flux analysis.Metabolic control analysis (MCA): Determination of Flux control coefficients, MCA of Linear and Branched pathways. (10 lectures)

Module: 4 Metabolic designs: Gene amplification, Gene-disruption, Randomized and targeted strain development. Metabolic Engineering in Practice: Actual examples from research and industrial biotechnology. (10 lectures)

Knowledge of stoichiometry and energetics of metabolism.

Learn the methods for metabolic characterizations

Learn the theory and methods of metabolic flux analysis

To apply metabolic design for research and Industrial Biotechnology

• Understand the basics of metabolic engineering and its terminologies
• Gain the knowledge regarding the techniques used in metabolomics
• Understand the methods and theory behind metabolic flux analysis
• Know the elements of metabolic design
• Understand the applications of metabolic engineering in research and industrial biotechnology

Stephanopoulas, G, et al, Introduction to Metabolic engineering – Principles and Methodologies, Elsevier Science

S. Y. Lee & E.T. Papoutsakis, Metabolic Engineering, Marcel Dekker, New York, 1999

R. Heinrich and S. Schuster, The Regulation of Cellular Systems, Chapman & Hall, 1996

E.O. Voit, Computational Analysis of Biochemical Systems, Cambridge University Press, 2000.