Syllabus

Module 1: Fundamentals of Bioseparation
Introduction to Bioseparation, Properties of Biomolecules
Module 2: Cell Disruption and Primary Recovery
Cell Disruption Techniques, Primary Separation Techniques
Module 3: Product Enrichment Methods
Precipitation Techniques, Extraction Methods, Membrane-Based Separation
Module 4: Chromatographic Techniques
Principles of Chromatography, different Chromatographic Methods: conventional and advanced, concepts of theoretical
Module 5: Advanced Separation and Polishing
Electrophoresis Techniques, Crystallization and Polishing, Product Stability and Formulation
Module 6: Case Studies and Emerging Trends
Industrial Applications, Scale up and its related problems, Emerging Technologies, Regulatory and Economic Aspects

1. Understand fundamental principles of bioseparation processes and their critical role in biotechnology and bioprocess industries.

2. Understand the physicochemical properties (such as size, charge, hydrophobicity, and stability) of biomolecules and choose separation techniques accordingly.

3. Evaluate and apply bio-separation techniques to purify biomolecules efficiently

4. Address real-world challenges in bioseparation by integrating process knowledge with regulatory and economic considerations.

CO1 Explain the importance of bioseparation processes and analyze the physicochemical properties of biomolecules that influence separation techniques.
CO2 Select and implement appropriate cell disruption and primary recovery techniques for efficient extraction of intracellular or extracellular biomolecules.
CO3 Apply advanced precipitation, extraction, and membrane-based methods to enrich and purify target biomolecules.
CO4 Utilize chromatographic and electrophoretic techniques to purify biomolecules to high standards and ensure stability during final product formulation.
CO5 Address industrial challenges related to scale-up, regulatory compliance, and economic feasibility while staying updated with emerging technologies in bioseparation.

P.A. Belter, E.L. Cussler and Wei-Houhu, Bioseparations – Downstream Processing For Biotechnology,, Wiley Interscience Pub

R. K. Scopes, Protein Purification: Principles and Practice,, Springer

R Ghosh, Principles of Bioseparation Engineering, World Scientific Pte. Ltd,

RG Harrison, PW Todd, SR Rudge. D Petrides, DP Petrides, Bioseparations Science and Engineering, Oxford University Press,