Syllabus

Module 1- Analysis of proteome (6 hours)
Introduction and scope of proteomics and genomics, Protein separation techniques, Isoelectric focusing (IEF), Two dimensional PAGE for proteome analysis, Image analysis of 2D gels, Introduction to mass spectrometry

Module 2- Protein identification strategies (5 hours)
Strategies for protein identification, peptide mass fingerprinting, Protein sequencing, Post translational modifications of proteins, Quantitative proteomics

Module 3- Applications of proteomics (5 hours)
Applications of proteome analysis to drugs, Protein-protein interaction (Two hybrid interaction screening), Protein chips and functional proteomics, Clinical and biomedical application of proteomics, Proteome database and data analysis

Module 4- Analysis of genome (7 hours)
Methods of preparing genomic DNA, DNA sequence analysis methods: Sanger Dideoxy method and Fluorescence method Gene variation and Single Nucleotide Polymorphisms (SNPs)

Module 5- Applications of genomics (7 hours)
Expressed sequenced tags (ESTs) Gene-disease association, Recombinant DNA technology: DNA cloning basics, Polymerase chain reaction, DNA fingerprinting, Human genome project and the genetic map, CRISPR-Cas9

Inferring the basic concepts of genomics and proteomics.

Listing and discussing the use of genomics and proteomics in human health.

Suggesting and outlining solution to theoretical and experimental problems in Genomics and Proteomics fields.

1) To understand various methods of analysis of proteome and genome.
2) To interpret data obtained through high throughput expression studies.
3) To learn different applications of proteomics and genomics in biotechnology and biomedical engineering.
4) To apply basic knowledge of genomics and proteomics to solve theoretical and experimental problems in biotechnology and biomedical engineering.

R.M.Twyman, Principles of Proteomics, BIOS Scientific Publishers, 2004.

Arthur M Lesk, Introduction to Genomics, Oxford University Press, 2007.

P. Michael Conn, Handbook of Proteomic Method, Humana Press, Totowa, New Jersay, USA, 2003.

L. Stryer, Biochemistry, W. H. Freeman and Co., New York, 2007.