Syllabus

Modeule1. Basics of DNA cloning: Enzymes and vectors (cloning and expression vectors) in gene cloning, methods of construction of rDNAs, methods of introduction of rDNAs into living systems, screening of rDNA clones. [9 hrs]
Module 2. Methods of DNA and protein analysis: Electrophoretic techniques, PCR (types), RFLP, DNA fingerprinting and footprinting, chromosome walking, blotting techniques, Native PAGE, SDSPAGE and two dimensional PAGE analysis of proteins, Western Blotting .[5 hrs]
Module 3. Construction and Screening of DNA libraries: Vectors used in the construction of cDNA versus genomic DNA libaries, Steps and enzymes involved, method of screening libraries, screening expression libraries, Positive selection and subtractive hybridyzation by identifying genes in complex genomes, DNA mapping and sequencing.[9 hrs]
Module 4. In vitro gene expression systems and protein engineering: Expression and purification of recombinant proteins in microbes. Phage display Insertional and deletion mutagenesis, Site directed mutagenesis, antisense and ribozyme technology, gene targeting. Proteome analysis, Protein arrays and their applications. [7hrs]
Module5. Transgenesis and genome editing: Genome Editing: ZFN, TELENS, CRISPR, Ethical and legal issues in rDNA technology.[6 hrs]

To impart training on fundamentals of gene manipulation using genetic engineering tools.

To provide a knowledge to use genetic engineering tools in analysing the manipulated DNA and proteins

To expose the students to the application of genetic engineering in biotechnological research.

To develop an understanding on the regulatory, ethical and legal aspects in genetic engineering.

1. To understand and choose the appropriate genetic engineering tool-box for the purpose of manipulating genes and genome.
2. To understand and identify the appropriate tool for the purpose of genome mapping and sequencing
3. To understand the production of industrial proteins using recombinant DNA technology and genome editing for clinical applications.
4. To understand and design experiments for Transgenesis
5. To learn and understand the bio-safety issues, regulatory bodies, ethical, legal and IPR issues in genetic engineering.

• Brown TA, Gene cloning and DNA analysis, Wiley-Blackwell, USA , ISBN: 978-1-4051-8173-0

• Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics, Wiley-Blackwell, USA , ISBN: 978-0632059546

• Pinkert CA, Transgenic animal technology, Academic press, Amsterdam. The Netherlands

• Cartwright EJ, ransgenesis techniques: Principles and Protocols. Methods in Molecular Biology 561, Springer Protocols, NY, USA