Course Details
Subject {L-T-P / C} : BM6672 : Plant Molecular Biology Laboratory { 0-0-3 / 2}
Subject Nature : Practical
Coordinator : Nivedita Patra
Syllabus
| Module 1 : |
Tissue culture media preparation, Callus induction, Hairy root induction, Plant genomic DNA isolation, RNA isolation, Reverse transcriptase PCR, Genetic transformation of plants effect of cultivation conditions, Elicitation of secondary metabolites, Permeabilization, In-situ product extraction, Immobilization of plant cells, Scale-up of plant cell cultivation in a bioreactor, batch kinetic study in bioreactor. |
Course Objective
| 1 . |
Introduction to basic plant tissue culture techniques |
| 2 . |
Training of plant molecular biology techniques |
| 3 . |
Application of plant biotechnology for large scale production of phytopharmaceuticals |
Course Outcome
| 1 . |
The students will be given hands-on training in plant tissue culture techniques. They will perform plant molecular biology techniques. They will apply the basic knowledge of plant tissue culture for bioprocess technology applications. |
Essential Reading
| 1 . |
Robert D. Hall, Methods in Molecular Biology, Volume 111, Cell Culture Protocols, Humana Press Inc , First Edition, 1999 |
Journal and Conferences
| 2 . |
World Ayurveda Congress and Arogya Expo |
| 1 . |
Theoretical and Applied Genetics, Springer-Verlag GmbH Germany |