Syllabus

Module 1: Quantitative estimation of biomolecules like proteins, nucleic acids, lipids, carbohydrates, Determination of DNA melting temperature

Module2: Denaturant mediated protein unfolding using fluorescence spectrophotometer, pH mediated protein unfolding using fluorescence spectrophotometer, Temperature mediated protein unfolding using fluorescence spectrophotometer, effects of pH on enzyme kinetics, effect of temperature on enzyme kinetics, effect of substrate concentration on enzyme kinetics

Module 3: Chromatographic separation of biomolecules Protein ligand/nucleic acid ligand interaction studies using biophysical methods.

Module 4: Physio-chemical and mechanical characterization of biomaterials, Develop a basic analytical tools using simple electronics

Techniques of studying protein biomolecules

Learn techniques to understand the nature of nucleic acids

Learn practical chromatographic techniques and its theory to separate and analyse analytes

Learn aspects of instrumentation of analytical techniques

1. Students will learn techniques for studying interaction of proteins.
2. Students will acquire knowledge for analyzing different biophysical properties of biomolecule
3. Gain expertise in separation of chemical and biological molecules
4. Acquire skills to characterize the materials and biomolecules
5. Gain knowhow to develop analytical tools for analysing a sample

J. Cazes, Analytical Instrumentation Handbook., CRC Press.