National Institute of Technology Rourkela

राष्ट्रीय प्रौद्योगिकी संस्थान राउरकेला

ଜାତୀୟ ପ୍ରଯୁକ୍ତି ପ୍ରତିଷ୍ଠାନ ରାଉରକେଲା

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Syllabus

Course Details

Subject {L-T-P / C} : BM6172 : Biomaterials and Tissue Engineering Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Amit Biswas

Syllabus

Module 1 :

1. Preparation of Porous 3D Scaffold by Freeze Gelation Method
2. Preparation of Porous 3D Scaffold by Freeze Drying Method
3. Fabrication of Nanofibrous Scaffold by Electrospinning
4. Evaluation of Swelling Behavior of Scaffolds
5. Assessment of Degradation Profile of 3D Porous Scaffold
6. Fabrication of 3D Scaffold Using Rapid Prototyping Technique
7. Measurement of Contact Angle for Wettability Analysis
8. Isolation of Mononuclear Cells from Peripheral Blood
9. Cell Viability Assessment Using Trypan Blue Exclusion Assay
10. Cell Viability Assessment Using Fluorescence Microscopy
11. Determination of Tensile Strength of Electrospun Scaffold
12. Static Cell Seeding on Scaffold for Tissue Engineering Applications
13. Anodic Oxidation of Ti-6Al-4V for Biomedical Applications
14. Rheological Characterization of Polymer Solution Using Cone and Plate Method
15. Evaluation of Wear Resistance of Implant Materials

Course Objective

1 .

Gains hands-on experience on various methods in biomaterials and tissue engineering

2 .

Understanding different physical and chemical properties of the biomaterials required in tissue engineering.

3 .

Fabrication, surface treatment and coating of different implant materials

4 .

Understand working principles of various characterization techniques and protocols used in biomaterials and tissue engineering

Course Outcome

1 .

-Demonstrate laboratory skills in fabrication and characterization of scaffolds for tissue engineering.
-Able to understand the need of different characterization techniques.
-Able to explain how composition, structure and treatment modify the mechanical properties.
-Able to describe the interactions of biomaterials with the biological environment
-Learn aseptic techniques in handling cell culture and tissue engineering.

Essential Reading

1 .

-Wujing Xian, -A Laboratory Course in Biomaterials, -CRC Press , -