Syllabus

1. Preparation of bacterial competent cells
2. Transformation of competent bacterial cells
3. Plasmid DNA isolation
4. Genomic DNA isolation
5. Bacterial cell lysis using sonication
6. DNA amplification using PCR
7. DNA separation using agarose electrophoresis
8. Ammonium Sulfate precipitation of proteins
9. Protein separation using SDS PAGE
10. Desalting of proteins using dialysis

To learn basic molecular biology techniques

To learn methods of gene transfer in bacterial cells

To learn gene amplification using PCR

To learn methods of isolation and analysis of biomolecules

1) Preparation and transformation of bacterial competent cells
2) Plasmid and Genomic DNA isolation
3) DNA amplification using PCR
4) Purification of biomolecules using various methods

G. Karp, Cell and Molecular Biology: Concepts and Experiments, Wiley