Syllabus

Module 1: Introduction to Recombinant DNA Technology
Brief history of recombinant DNA Technology, Relevance and impact of rDNA technology, Applications of Recombinant DNA Technology including I health, agriculture, food process, environment and energy sectors.
Module 2: Tools used in RDT
Enzymes used in gene manipulation, host systems and, Gene isolation, cloning and expression, DNA sequencing, oligonucleotide synthesis, Southern and Northern hybridization, FISH, PCR, RAPD, RFLP, DNA fingerprinting and their applications for diagnosis of disease,
Module 3: Advanced Techniques in RDT
Site-directed mutagenesis, Gene silencing, Gene transfer technologies, Gene therapy, Functional genomics: DNA chips and microarray gene screen technology transgenic animals and gene knockout techniques. Genome sequencing projects, advancement of genome sequencing using Next generation Sequencing (NGS).
Module 4: Study of emerging genome editing tools
Zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and RNA-guided engineered nucleases (RGENs) derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system.
Module 5: Ethical and Biosafety Considerations
Ethical issues in RDT, Regulatory frameworks for genetically modified organisms (GMOs), Ethical clearances for recombinant products.

To gain detailed knowledge about molecular biology

To learn the real application of RDT in different area

To learn advancement of the recombinant DNA technology

To learn ethical issues regarding recombinant products

1. Technical skills and knowledge development on versatile techniques in rDNA technology
2. Thorough understanding about the real applications of rDNA Technology
3. Gaining insight in the new advancements of the rDNA techniques and their field of application in real context
4. Expertise in designing and conducting experiments involving genome editing and manipulation
5. Development of awareness related to ethical issues of RDT

Brown TA, Gene cloning and DNA analysis, Wiley-Blackwell, USA.

Primrose SB, Twyman RM, Old RW, Principles of gene manipulation and genomics., Wiley-Blackwell, USA.

Reece RJ, Analysis of genes and genomes, Wiley, USA

Lewin B., Genes IX, Jones and Bartlett, USA