National Institute of Technology Rourkela

राष्ट्रीय प्रौद्योगिकी संस्थान राउरकेला

ଜାତୀୟ ପ୍ରଯୁକ୍ତି ପ୍ରତିଷ୍ଠାନ ରାଉରକେଲା

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Syllabus

Course Details

Subject {L-T-P / C} : BM6571 : Analytical Techniques in Bioengineering Laboratory { 0-0-3 / 2}

Subject Nature : Practical

Coordinator : Kunal Pal

Syllabus

Module 1 :

At least 10 experiments from the following list of experiments will be carried out:
1. Analytical Bioengineering Foundations: Good Laboratory Practices, Quantitative Accuracy, Preparation of a 1 % (w/v) Sucrose Solution, and Introduction to Data Analysis and Statistical Interpretation of Data
2. Determination of Salicylic Acid Concentration by UV–Visible Spectroscopy
3. Measurement of Color Attributes by using a Reflective Colorimeter
4. Qualitative Evaluation of Color and Spectral Characteristics of Biomaterials Using Hyperspectral Imaging
5. Identification and Characterization of Functional Groups using ATR–FTIR Spectroscopy
6. Characterization of Electrical Characteristics of Biomaterials Using Electrical Impedance Spectroscopy (EIS)
7. Assessment of the Viscoelastic Properties of Biomaterials Through Stress Relaxation Analysis Using a Texture Analyzer
8. Optical Characterization of Biomaterials Using Brightfield and Polarized Microscopy
9. Identification and Rf Analysis of Individual Components from a Mixed Dye Solution by Ascending and Circular Paper Chromatography
10. In-Vitro Evaluation of Tablet Disintegration Performance Using a Standard Disintegration Tester
11. In-Vitro Determination of Drug Release Behavior Using a Tablet Dissolution Apparatus
12. Quantitative Assessment of Sugar content by Refractometric Analysis
13. Optical Characterization of Emulgels using Fluorescence Microscopy
14. Study of Dehydration Kinetics of Biomaterials Using Empirical Mathematical Models
15. Measurement of Viscosity of Liquids using Ostwald Viscometer

Course Objective

1 .

The main objective of the course is to provide advanced hands-on training to students in the operation and application of modern analytical instruments used in bioengineering, enabling accurate quantitative and qualitative characterization of biomaterials and biological systems.

2 .

The course aims to develop strong competence in Good Laboratory Practices (GLP), experimental design, data acquisition, and statistical validation, ensuring accuracy, precision, reproducibility, and scientific integrity in analytical bioengineering experiments.

3 .

The course is designed to enhance students’ ability to interpret, analyze, and scientifically report experimental data using appropriate graphical, statistical, and analytical tools, thereby preparing them for research, industrial, and regulatory laboratory environments.

Course Outcome

1 .

Apply principles of GLP to ensure accuracy, precision, reproducibility, and safety in analytical experiments

2 .

Demonstrate hands-on proficiency in quantitative and qualitative analytical techniques

3 .

Perform data analysis and statistical interpretation to validate experimental outcomes

4 .

Integrate analytical bioengineering concepts using modern instrumentation

5 .

Develop the ability to interpret and report experimental results effectively with appropriate graphical, statistical, and scientific presentation skills following GLP and ethical standards

Essential Reading

1 .

Douglas A. Skoog, F. James Holler & Stanley R. Crouch, Principles of Instrumental Analysis, Cengage Learning India Pvt. Ltd. , ISBN-13: 978-9353506193

2 .

Gary D. Christian, Purnendu K. Dasgupta & Kevin A. Schug, Analytical Chemistry, Wiley , ISBN-13: 978-0470887578

Supplementary Reading

1 .

Hobart H. Willard, Lynne L. Merritt Jr., John A. Dean & Frank A. Settle Jr., Instrumental Methods of Analysis, CBS Publishers & Distributors , ISBN-13: 978-8123909431

2 .

Gurdeep R. Chatwal & Sham K. Anand, Instrumental Methods of Chemical Analysis, Himalaya Publishing House , ISBN-13: 978-9351420880

Journal and Conferences

1 .